Composite

Part:BBa_K1197015:Design

Designed by: Alişan Kayabölen   Group: iGEM13_METU_Turkey   (2013-10-02)


Catalytically active CYP6G1 coding device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 767
    Illegal BamHI site found at 3178
    Illegal XhoI site found at 1435
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1168
    Illegal BsaI.rc site found at 3700


Design Notes

Promoter and RBS are specific for expression and signal peptide is specific for secretion in B. subtilis.


Source

CYP6G1 comes from Drosophila melanogaster genome and fCPR comes from Musca domestica.

References